Journal: medRxiv
Article Title: Single-Cell Analysis Reveals Inflammatory–Immunosuppressive Niches in Daratumumab-Resistant Primary AL Amyloidosis
doi: 10.64898/2026.03.28.26349317
Figure Lengend Snippet: (A) UMAP of BM PCs and microenvironment cells in the whole BM samples. (B) PC–centered immune cell communication algorithm to compute the hematologic response-related ligand-receptor cell-cell communication probabilities. (C) Dot plot showing response-related ligand–receptor interactions between PCs and microenvironment immune cells grouped by pre/post-treatment and hematologic response. (D) HLA-E–KLRK1 signaling representing non-classical MHC class I–mediated ligand–receptor pairs associated with hematologic response. (E) Prostaglandin pathway–mediated ligand–receptor pairs associated with hematologic response. (F) Multiplex immunofluorescence validation of prostaglandin pathway activation in BM tissue sections. Colors: DAPI (blue), CD138 (red), CD14 (green), PTGS2 (yellow), PTGER2/4 (cyan), PTGES3 (white), PTGES2 (orange). Scale bars: column 1-4 (20 μm); column 5 (100 μm).
Article Snippet: PC enrichment was performed using CD138 + Magnetic Beads (Miltenyi Biotec, Bergisch Gladbach, Germany; Cat# 130-051-301), MS columns (Cat# 130-042-201), and a MiniMACS separator (Cat# 130-042-102).
Techniques: Multiplex Assay, Immunofluorescence, Biomarker Discovery, Activation Assay